I recently did a lab where we were using spectrophotometers to determine the glucose concentration of two patient plasma samples, to determine whether or not they had Diabetes Mellitus. We were reacting the samples with Glucose Oxidase and Peroxidase which produces a pink-red colour and then using the spectrophotometer to find their absorbance. First we had to use known concentrations of glucose to gain absorbances to produce a calibration curve. Then we were instructed to do 10 replicates of a "Plasma Pool" sample, and find their concentrations - but I don't know what this is for! I know I need to work out the average glucose concentration, the standard deviation, and then the coefficient of variation of the Plasma Pool samples. And I have to write about the accuracy/precision of the procedure, so I assume that is the point of the Plasma Pool samples - to test the accuracy. But what is "Plasma Pool" itself?? Also, if anyone knows about the Glucose Oxidase test itself I just want to check - the deeper the colour, the more glucose there is? Is that right? If anyone can help it would be greatly appreciated! Thank you!
Biology - 1 Answers
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Pooled plasma is plasma from multiple individuals. It is often used as a reference standard and assayed using a "gold standard" test. Glucose oxidase produces hydrogen peroxide which can be used to develop color in a variety of reagents. Not knowing what color reagent or protocol you are using, all I can say is that a deeper color should indicate more reaction.
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